Advances in Antibody Phage Display – A review

Antibody phage technology greatly facilitates the isolation of good-quality monoclonal antibodies to virtually any target antigen. Large combinatorial phage display libraries of human antibodies are routinely being used for the identification of antibody candidates for clinical applications. However, preclinical studies in rodents would benefit from the availability of good-quality single-pot mouse synthetic naïve antibody libraries, which at present are not available. Such libraries would be particularly useful for the generation of murine antibodies against self or highly conserved antigens or in case of highly toxic or deadly pathogenic antigens which do not allow animal immunization.